After optimizing prokaryotic expression conditions, we determined the optimum inducement time and concentration of IPTG.
通过优化原核表达条件，确定了原核表达的最佳诱导时间和诱导剂浓度。
The induction temperature, induction time, and IPTG concentration were also optimized by a series of experiments. Further purification modes of this protein were also explored.
同时还进行梯度实验分别对诱导的温度、时间和IPTG诱导时菌体浓度进行优化，并对蛋白的纯化方案进行摸索。
The expression condition of plasmid pET-tmcC has been explored, but at all the testing temperature, IPTG concentration and different hosts protein expression was not found.
将重组质粒转化至大肠杆菌宿主中，对目的蛋白进行诱导表达，并对诱导表达条件如温度、IPTG浓度等进行了优化。
应用推荐