杨蓉 脑神经嵴干细胞体外培养方法的研究进展 关键词】 脑神经嵴； 干细胞； 体外培养 [gap=612]Key words] cranial neural crest； stem cell； in vitro culture
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cranial neural crest cells 颅神经嵴细胞 ; 其中脑神经嵴细胞 ; 在脑神经嵴细胞 ; 脑神经嵴细胞
cranial neural crest stem cell 神经嵴干细胞 ; 颅神经嵴干细胞
Cranial neural crest cell 神经嵴细胞 ; 颅神经嵴细胞
RESULTS: Cranial neural crest cells were cultured successfully. The shape of the cells were fibroblast-like.
结果：成功地培养出小鼠的颅神经嵴细胞，其形态类似成纤维样细胞。
METHODS: cranial neural tubes were dissected from 8.5 day mouse embryo using trypsin digestion and explanted in culture dishes. Cells dissociated from the neural tubes were cranial neural crest cells.
方法：采用胰酶消化法分离小鼠胚胎第8 . 5天的颅神经管，从小鼠颅神经管中游离出来的细胞即为颅神经嵴细胞。
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