采用比较简便的提纯方法,从300g人脑中得到凝胶电泳纯的肌酸激酶BB同工酶(Creatine kinase BB isoenzyme,CK-BB)24mg,比活力高达1104u/mg蛋白,酶活力回收66％。同时,制备兔抗人CK-BB抗血清。
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采用比较简便的提纯方法,从300g人脑中得到凝胶电泳纯的肌酸激酶BB同工酶(Creatine kinase BB isoenzyme,CK-BB)24mg,比活力高达1104u/mg蛋白,酶活力回收66％。同时,制备兔抗人CK-BB抗血清。
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human creatine kinase bb isoenzyme 人肌酸激酶bb同工酶
Methods Serum cytosolic enzyme creatine kinase (CK) and its brain band isoenzyme (CK BB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) were measured with autobiochemical analyzer.
方法采用全自动生化分析仪检测血清肌酸激酶（CK）、肌酸激酶脑型同工酶（CK-BB）、乳酸脱氢酶（LDH）以及天门冬氨酸氨基转换酶（AST）的含量。
Results:The CK_MB activity in all these 30 cases was normal and BB isoenzyme of creatine kinase(CK_BB) or some other abnormal CK isoe.
结果：经CK同工酶电泳证实，此组患者CK_MB活性实际为正常，但出现脑型肌酸激酶同工酶（CK_BB）或异常CK同工酶带。
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