AIM: To observe whether antigens can induce the restrained peripheral lymphocytes response under mixed culture in vitro, and establish a model of restrained lymphocytes response in vitro.
目的：观察在体外混合培养条件下，抗原能否诱导外周淋巴细胞反应抑制，建立体外淋巴细胞反应抑制模型。
Methods Co culture systems of RPE cells with antigen specific t lymphocyte lines and resting t lymphocytes were established in vitro.
方法建立RPE与抗原特异性T淋巴细胞系和静止淋巴细胞之间的共培养系统。
The separation of tumor-infiltrating lymphocytes (TIL) from tumor mass of B16 melanoma was achieved by discontinuous density gradients centrifugation and the in vitro culture of TIL were investigated.
本文运用不连续密度离心的方法从鼠b 16黑色素瘤体中分离到肿瘤浸润性淋巴细胞(TIL)，并对TIL的体外培养的条件及细胞性质进行了研究。
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