本研究尝试应用胎牛成纤维母细胞（fetal fibroblast cell）及 卵丘细胞 （ cumulus cell ）作为供核细胞，并以体外成熟且业经去核之牛卵母细胞作为受核细胞进行核移置试验，包括比较：1.
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layers of cumulus cell 卵丘细胞层多少
pig cumulus cell enclosed oocyte 猪卵丘细胞
cumulus cell nuclear transplantation 卵丘细胞核移植
cumulus granulosa cell 丘颗粒细胞
When oocytes were matured at 37-39℃,the appearance of cumulus cell had protective effect on cleavage and blastocyst rate,the development rate of DOs significantly decreased compared to COCs.
4.当卵母细胞在37-39℃成熟培养时,卵丘细胞对卵母细胞的发育有一定的保护作用,DOs的发育能力显著低于COCs。
参考来源 - 小鼠卵母细胞体外成熟过程中热应激对胞质成熟的影响The reconstruction embryo cleavage and blastocyst rate by the frozen cumulus cell or by the non-frozen group were not significantly different((78.61% vs89.12%,29.42% vs33.65%) (P> 0.05).
9.颗粒细胞的冷冻处理组进行核移植得到重构胚的分裂率和囊胚率与不冷冻组之间没有显著差异(78.61% vs89.12%,29.42% vs33.65%)(P>0.05)。
参考来源 - 牛手工体细胞克隆技术的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
In general idea, cumulus cell is the best nuclear donor cell for reprogramming.
一般来说，颗粒细胞作为核供体最易被核重编程。
The results indicated that there were no notable difference between granulosa cell monolayers group and cumulus cell monolayers group for the cleavage, blastocyst and hatched rate (P>0.05).
结果表明，就卵裂率、囊胚率和囊胚孵出率而言，壁颗粒细胞单层组与丘颗粒细胞单层组之间无显著差异（P>0.05）；
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