Objiective to probe into the methods of tissue-engineering bone construction in vitro by building osteoblasts-decalcification bone scaffolds complex and observing its ability of inducing osteogenesis.

目的：建立成骨细胞-脱钙骨支架复合物，观察其诱导成骨能力，从而探寻组织工程化骨的体外构建方法。

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Methods Hydrochloric acid (HCL) decalcification method and TCA-acetone precipitation method were separately used for bone protein extraction. The efficiencies of these two methods were compared.

方法 采用盐酸脱钙法和三氯醋酸-丙酮沉淀法，分别用于骨组织蛋白的提取，并对两种方法的提取效率进行对比。

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