differentiation plantlet from callus 愈伤分化再生阶段
With this medium, soft, transparent callus can be induced from immature embryos of embryo selection or tissue and organ differentiation stages. The induction frequency was 2-6%.
以该配方诱导幼胚,处于胚胎选择阶段和组织与器官分化阶段的幼胚,能诱导出松软、晶莹透明的愈伤,诱导频率为2-6%。
The explants from immature embryo of 4 wheat varieties were cultured in vitro to study the affecting factors on callus induction and differentiation.
以4个小麦品种的幼胚为外植体,通过脱分化和再分化培养,研究了影响小麦幼胚愈伤组织诱导和再分化的因素。
In callus subculture, increase in 2, 4-d content kept the callus from differentiation, though, high concentration of 2, 4-d did harm to the callus.
在继代过程中,适当提高2,4 - D浓度可抑制愈伤的分化,但为防止高浓度2,4 - D对愈伤的伤害,分化趋势一旦控制应立即转入低浓度2,4 - D的继代培养基中。
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