Use of most molecular marker systems depends on the polymerase chain reaction (PCR), which is an important technique for amplifying specific DNA sequences.
大多数分子标记体系的利用都取决于聚合酶链反应(PCR),这是一项扩增特定DNA序列的重要技术。
Methods: Polymerase chain reaction (PCR) was adopted in detection of DNA of mycobacterium tuberculosis from peripheral blood and sputum in 64 tuberculosis patients.
方法:采用聚合酶链反应(PCR)技术,检测了64例肺结核患者的外周血与痰标本结核杆菌dna。
Meanwhile, biochemical reaction, coagglutination test, metabolism-inhibition test, polymerase chain reaction (PCR), and DNA sequence assay were employed to identify those positive cultures.
阳性培养物用生化反应、协同凝集试验、代谢抑制试验、聚合酶链反应和DNA测序来加以鉴定。
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