The hybridization event was recognized by the different FRET efficiencies due to different interaction abilities of single-stranded and double-stranded DNA with the polymer.

由于单股和双股DNA与聚合体之间具有不同的相互作用能力，因而可通过不同荧光能量共振转移效率识别杂交事件。

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It operates in a similar fashion to DNA polymerase in that it denatures locally or opens up the double stranded DNA, but it's different in that it creates a new polymer from the DNA template in the language of RNA, using RNA nucleotides and not DNA nucleotides.

它的作用机制类似于DNA聚合酶,在局部使DNA双链变性,解旋,但不同的是,它合成了一个基于DNA模板的,使用RNA编码语言的新聚合物,即使用核糖核苷而非脱氧核糖核苷

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