The phosphorylation of STAT5 and its location were examined by double immunohistochemical staining and the binding of STAT5 with DNA was determined by electrophoretic mobility shift assay (EMSA).

用双染免疫组化法检查脾细胞中STAT 5磷酸化与STAT 5抗原的定位。用电泳迁移率变动分析(EMSA)测定STAT 5与DNA探针的结合力。

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Methods Immunohistochemistry and electrophoretic mobility shift assay (EMSA) were used.

方法采用免疫组化法及凝胶电泳迁移率实验。

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First, we carried out Electrophoretic Mobility Shift Assay (EMSA) to investigate the binding difference of SNPs and transcription factors.

首先使用凝胶迁移检测和疾病关联的SNP是否会影响某些转录因子的结合。

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