BALB/c鼠肝脏枯否细胞不同分离培养方法的比较-临床医学论文-论文联盟 关键词】 枯否细胞;分离;鉴定;酶消化法 [gap=842]Key words] Kupffer Cells; Isolation; Identification;Enzyme Digestion
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关键词：抽提 酶切 转膜 杂交 [gap=4325]Key word: extraction, enzyme digestion, transfer membrane, hybridization ...
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用酵素切割 (enzyme digestion) 将细胞从培养 基洗出并去除死细胞，将细胞稀释之后置入新鲜的培 养基培养七日，然后选单一菌落 (single colony) 培养七至九 ..
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restriction enzyme digestion 限制酶切割 ; 酵素切割
limited enzyme digestion method 限制性酶解法
Cold enzyme digestion 冷酶消化法
2、The most quantity of active cells was obtained in 4th h. . 3、Fibroblast can be purified by enzyme digestion and different adherence time.
胶原酶消化法在4小时时获得的有活性细胞量最多。
参考来源 - 应用组织工程材料栓塞动脉瘤的实验研究Some bacteria in nature can keep occurring enzyme digestion process with surrounding medium through its own life activity, which, after a long-time accumulation, enable to agglutinate those precipitated loosen classic substances into solid rock.
自然界中某些微生物可以通过其自身的生命活动,与周围环境介质之间不断发生酶化作用,逐渐矿化形成一种胶结物质——方解石。 再经过漫长时期的累积,最终可以将自然界中沉积的疏松碎屑物质胶结形成坚硬的岩石。
参考来源 - 碳酸盐矿化菌研究·2,447,543篇论文数据，部分数据来源于NoteExpress
The method of explanted tissue culture is better than the method of enzyme digestion for the primary cells.
组织块培养法对于原代细胞的培养要优于酶消化法。
Conclusion The enzyme digestion procedure is a stable and reliable method to obtain bovine retinal pericytes.
结论：酶消化法原代培养牛视网膜周细胞是一种稳定、可靠的方法。
Results The constructed vectors of EBO-WT and EBO-G87 were identified by restriction enzyme digestion and nucleotide sequencing.
结果构建的EBO G87和EBO WT重组载体经内切酶双酶切鉴定及核苷酸序列测定证实。
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