The HBV markers and HBV DNA in poultry sera, Yolk and bovine milk whey were detected by reversed passive hemagglutination assay (RPHA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR).
应用反向间接血凝试验（RPHA）、酶免疫测定（EIA）和聚合酶链反应（PCR）测定了家禽血清、卵黄和牛乳清中HBV标志物及人HBV-DNA。
Methods Hepatitis B serum markers and Pre-S2 antigens were tested by enzyme-linked immunosorbent assay and HBV DNA was detected by fluorescent quantitative-PCR in 982 hepatitis B patients.
方法 用酶联免疫吸附试验对982例乙肝患者血清标志物和乙肝病毒前S2抗原进行检测；并用荧光定量PCR法对其进行HBV-DNA检测。
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