Methods: We selected the best medium fitted for es cells culture, and analyzed the neural differentiation of es cells by comparing increased number of viable cells among different serum-free medium.

方法：比较ES细胞在不同培养基中的生长情况，分析ES细胞在不同时间分化形成神经细胞的比例。

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Feeder layer cells and suitable culture medium that supplemented with cytokines of promoting proliferation or restraining differentiation were crucial to keep chicken es in an undifferentiated state.

为维持细胞在培养过程中的未分化状态，需要采用饲养层细胞培养，同时设计合理的培养液配方并添加多种抑制分化或促进增殖的细胞因子。

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Embryonic stem cells (ES cells) are unique cell populations with the ability of self-renewal, unlimited proliferation, multipotent differentiation and maintaining their undifferentiated state.

胚胎干细胞(ES细胞)具有自我更新、无限增殖、保持未分化状态和向所有三个胚层分化的潜能。

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