...实时荧光定量PCR；卫生检疫；检测方法 [gap=1618]Key words] Acidithiobacillus ferrooxidans； Fluorescent quantitative real-time PCR； Healthy quarantine；Detection method ..
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...列设计引物和TaqMan荧光探针,经优化反应条件,建立了M.suis的TaqMan荧光定量PCR检测方法(fluorescent quantitative real-time PCR,FQ-PCR):进行了FQ-PCR检测方法的敏感性、特异性和重复性实验,并对疑似M.suis感染临床抗凝全血样品进..
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real-time fluorescent quantitative PCR 实时荧光定量PCR ; 荧光定量PCR技术 ; 肝脏损害
real-time fluorescent quantitative RT-PCR 实时荧光定量RT
fluorescent real-time quantitative pcr 实时荧光定量pcr技术
quantitative real-time fluorescent pcr 实时荧光定量pcr
the real-time fluorescent quantitative pcr 实时荧光定量pcr
nested-quantitative real-time fluorescent PCR 巢式
real-time fluorescent quantitative pcr technique 实时荧光定量pcr技术
quantitative real-time fluorescent pcr techniques 实时荧光定量pcr技术
Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.
实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。
Objective:To set up a real-time fluorescent quantitative PCR assay for rapid detection of norovirus(NV) RNA in acute gastroenteritis.
目的：建立诺如病毒的荧光定量PCR检测方法，应用于急性胃肠炎的快速检测。
Methods:Viral RNA Was extracted from feces. 92 samples were detected by real-time fluorescent quantitative PCR and Enzyme-linked Immunosorbent Assay(ELISA).
方法：提取粪便中的RNA，用实时荧光PCR方法对丽水市急性胃肠炎的92份标本进行检测，并与常规ELISA检测结果比较。
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