Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.

实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。

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Objective:To set up a real-time fluorescent quantitative PCR assay for rapid detection of norovirus(NV) RNA in acute gastroenteritis.

目的：建立诺如病毒的荧光定量PCR检测方法，应用于急性胃肠炎的快速检测。

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To develop a fluorescent MGB probe real time PCR platform for detection the mutation of phenylalanine hydroxylase gene in patients with phenylketonuria.

探讨荧光mgb探针实时P CR技术检测经典型苯丙酮尿症的基因突变。

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Real-Time Polymerase Chain Reaction (RT-PCR) uses nanoscaled fluorescent probes that allow the tracking of the progress of the amplification from the start.

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