Site-directed mutagenesis is one of the key methods used to study gene function.
定点突变是研究基因功能的一种关键方法。
Many research groups successfully rely on whole-gene random mutagenesis and recombination approaches for the directed evolution of enzymes.
许多研究小组利用整基因随机突变形成和重组方法，已成功的进行了酶的定向进化。
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
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