用倒置显微镜观察Transwell小室背面的细胞数并分区计数细胞个数；取下小室的膜做苏木素一伊红(Haematoxylin and Eosin，HE)染色，在光镜下计数小室背面的细胞数。
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MAIN OUTCOME MEASURES: Brain tissue pathomorphology was measured by Haematoxylin and Eosin Staining.
主要观察指标：苏木精-伊红染色观察脑组织病理形态学改变。
The cells stained with haematoxylin and eosin displayed typical tardus neuron cellular necrosis in pallium and hippocampal CA1.
缺血再灌注组脑皮层及海马CA1区苏木精-伊红染色示典型的迟发性神经元坏死。
METHODS: A asthmatic model was established and inflammation changes of lung tissues were examined by haematoxylin-eosin staining.
方法：建立大鼠哮喘模型，苏木素-伊红染色观察肺组织炎症改变；
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