方法应用高分辨率溶解曲线（high resolution melting curve, HRM）技术对432例EM患者和499例无EM的妇女CYP17基因5’端非翻译区rs743572位点多态性（...
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Objective: To evaluate the feasibility and sensitivity of detecting the mutations of KRAS in colorectal carcinoma patients with high resolution melting curve analysis.
目的：探讨利用新的高分辨率熔解曲线分析法检测结直肠癌中KRAS基因突变的可行性。
Conclusions: The new high resolution melting curve analysis is more efficient, more convenient than direct sequencing. Moreover, it is a low-cost test, which is suitable for clinic test.
结论：新的高分辨率熔解曲线分析法在检测KRAS基因突变时比直接测序法具有耗时短，易操作的优点，并且检测成本低廉，适合在临床开展。
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