Conclusion the expression of TIMP-1, -2 in human dental pulp cell and tissue is detected, and this provides the basis for further studying on the mechanism of TIMP in dental pulp.
结论TIMP 1、2在人牙髓成纤维细胞及牙髓组织均有表达，为进一步研究TIMP在牙髓组织中的作用机理提供实验依据。
Methods In vitro study: pulp fibroblasts were collected and cultured from healthy human dental pulp by tissue explant method.
方法体外细胞实验：以组织块法体外培养人牙髓成纤维细胞，传代备用。
CONCLUSIONS: tissue explant method was superior to tissue explant-collagenase digestion method for the culturing of human dental coronal and root pulp cells.
结论：在对根、冠髓细胞的原代培养中，组织块法优于组织块酶解法。
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