...型C-kit cDNA得到突变型C-kit cDNA,与pcDNA3重组成真核表达质粒,并用脂质体法稳定转染人胚胎肾细胞(human embryonic kidney cell,HEK)293细胞系,G418加压筛选出阳性克隆;用Western印迹法检测转染细胞KIT蛋白表达,绘制细胞...
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...型C-kit cDNA得到突变型C-kit cDNA,与pcDNA3重组成真核表达质粒,并用脂质体法稳定转染人胚胎肾细胞(human embryonic kidney cell,HEK)293细胞系,G418加压筛选出阳性克隆;用Western印迹法检测转染细胞KIT蛋白表达,绘制细胞...
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人胚肾细胞 hck cell; human embryonic kidney cell 白细胞条件培养基 leucocyte-conditioned medium ..
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The expression of Lipocalin-2 gene was detected by RT-PCR and florescence observation. The effects of lipocalin-2 on growth of human embryonic kidney cell was observed by using recombinant lipocalin-2 and pEGFP-C1 transfection.
将真核表达质粒pEGFP-C1用脂质体转染法稳定转染人胚肾细胞,通过荧光观察,RT-PCR鉴定该基因的表达。
参考来源 - Lipocalin·2,447,543篇论文数据，部分数据来源于NoteExpress
Immortalized human embryonic kidney cell line HEK293 and the human embryonic lung fibroblasts HELF were used as positive control and negative control, respectively.
同时以端粒酶阳性的永生化人胚肾成纤维细胞HEK293作为阳性对照，以端粒酶阴性的人胚肺成纤维细胞HELF作为阴性对照。
Conclusion the N-GSLs in human embryonic kidney tissue seemed to be closely related to the growth and differentiation of the cell, and globoside may be the marker of normal kidney tissue.
结论中性糖脂参与了胚胎肾组织的成熟与分化过程，中性糖脂中长糖链红细胞糖苷脂与肾组织的恶变可能有关。
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