Objective To isolate and culture human neural stem cells from elder embryonic brain.
目的从大胚龄人胚脑中分离培养并鉴定神经干细胞。
Objective To study the conditions of in vitro culture and the features of differentiation of neural stem cells from human embryonic hippocampus.
目的研究人胚胎海马神经干细胞体外长期培养的条件和其在自主分化条件下的分化能力和分化特点。
Methods The serum free culturing technology was used to isolate, culture and pass neural stem cells from embryonic human hippocampus.
方法从人胚胎海马区分离神经干细胞，采用无血清培养基，进行体外扩增培养、传代。
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