AIM: the key of the human embryonic stem cell culture is to guarantee the totipotency and inhibit spontaneous differentiation.
目的：人胚胎干细胞传代培养的关键是抑制其自发分化、保证细胞的全能性。
To investigate and utilize human embryonic stem cell, establishment of a perfect culture system is crucial.
建立一个理想的人胚胎干细胞培养系统是研究和利用这种具有巨大潜力细胞的首要条件。
Objective To establish a culture system for human embryonic stem cell and label the hES cells with GFP by lentivirus.
目的稳定培养人胚胎干细胞，并通过慢病毒载体对其进行绿色荧光蛋白标记。
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