...金属螯合亲和层析；类人胶原蛋白；纯化 [gap=1069]Key words: protein engineering; immobilized metal ion affinity chromatography; recombinant human-like collagen; purification ..
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...层析(Metal Chelate Affinity Chromatographic,MCAC),又称固定金属离子亲和层析(Immobilized Metal Ion Affinity Chromatography,IMAC),是一种选择性好、操作简单的蛋白质分离方法。
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近年来, 一些富集方法不断改进及涌现，如抗体法、固相金属离子亲和色谱(Immobilized metal ion affinity chromatography，IMAC)、金属氧化物/氢氧化物亲和色谱(Metal oxide/hydroxide affinity chr...
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...层析(Metal Chelate Affinity Chromatographic,MCAC),又称固定金属离子亲和层析(Immobilized Metal Ion Affinity Chromatography,IMAC),是一种选择性好、操作简单的蛋白质分离方法。
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Immobilized metal ion affinity chromatography sorbents were prepared for purification of reduced glutathione (GSH) using chitosan as substrate.
制备了以壳聚糖为载体的金属螯合亲和色谱分离纯化还原型谷胱甘肽(GSH)。
By immobilized metal affinity chromatography, ion-exchange chromatography and hydrophobic interaction chromatography, the recombinant Gln49-PLA2 was purified with 91% purity.
通过固定化金属亲和层析、阴离子交换、疏水层析融合蛋白得到了纯化，其纯度可达91%。
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