Methods Flow cytometry and immunofluorescence staining technique were used.
方法：采用细胞免疫荧光染色技术和流式细胞术。
METHODS Immunofluorescence histochemical double staining technique was used and the staining results were observed with confocal laser scanning microscope.
方法免疫荧光组织化学双重染色，染色结果在激光共聚焦扫描显微镜下观察。
Further, he staining and immunofluorescence technique were used for morphometric analysis of islets in normal and HD mice.
并应用HE染色和免疫荧光技术分析正常和HD小鼠胰岛形态学差异。
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