Objective:Analyzing the affecting factors of forming competent cells of Escherichia coli JM109 to increase transformation efficiency.
目的:分析大肠杆菌JM109感受态形成因素,提高转化效率。
The reason for this transformation is that intra and inter-business electronic links greatly increase the efficiency of production, planning, the collecting of information and data exchange.
其原因就是企业内部和企业之间的电子网络极大地提高了生产、规划、信息收集以及数据交换的效率。
A significant increase of transformation efficiency was revcaled with the homologous plasmid isolated from recipient itself since the effects of host restriction and modification werc avoided.
来自受体菌自身的同源质粒,因克服了宿主的限制一修饰性,可以极显著地提高电转化效率。
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