The obtained cells were observed using transmission electron microscopy(TEM), detected with alizarin red staining and Gram staining, and identified by indirect immunofluorescence staining .
运用透射电镜、钙染色和革兰染色等手段观察其形态特征，采用单克隆抗体间接免疫荧光染色进行细菌鉴定。
Objective:To study molecule in basement membrane by electron microscopy and indirect immunofluorescence in patients with inherited epidermolysis bullosa.
目的： 通过透射电镜和免疫荧光研究先天性大疱性表皮松解症患者的基底膜带分子。
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