METHODS: The expression of TRAIL on K562 cells was detected by indirect immunofluorescence staining and flow cytometry.
方法：采用间接免疫荧光流式细胞术，分析K562细胞表面TRAIL分子的表达。
EGF receptors on the cell membrane were observed under a laser scanning confocal microscope after indirect immunofluorescence staining.
上述处理后的细胞经间接免疫荧光染色标记后，用激光共聚焦显微镜观察其细胞膜egf受体的聚簇现象。
The obtained cells were observed using transmission electron microscopy(TEM), detected with alizarin red staining and Gram staining, and identified by indirect immunofluorescence staining .
运用透射电镜、钙染色和革兰染色等手段观察其形态特征，采用单克隆抗体间接免疫荧光染色进行细菌鉴定。
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