The mutant was overexpressed in Escherichia coli BL21(DE3) by IPTG induction.
IPTG诱导突变体在大肠杆菌BL21（DE3）中高效表达。
The induction temperature, induction time, and IPTG concentration were also optimized by a series of experiments. Further purification modes of this protein were also explored.
同时还进行梯度实验分别对诱导的温度、时间和IPTG诱导时菌体浓度进行优化，并对蛋白的纯化方案进行摸索。
The modified gene was expressed by IPTG induction and purified by affinity chromatography and cleavage in place.
IPTG诱导该基因的表达，亲和层析和原位裂解法纯化表达产物。
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