METHODS Acidic dye colorimetry with bromothymol blue as ion pair reagent was employed to determine the alkaloids in samples.
方法用溴麝香草酚蓝同川芎生物碱形成稳定的有色离子对酸性染料比色法测定总生物碱的含量。
Results: After inspection the factors of different ion pair reagent and ionic strength which effect the system peak and the system peak effect analytic peak, the original method was improved.
结果:在考察了不同离子对试剂和离子强度等因素对色谱系统中系统峰的形状和保留值的影响及系统峰对被分析物色谱峰形的影响后,对原先的方法进行改进。
Products of the reaction mixtures were analyzed by ion pair reversed phase HPLC with SDS as the ion pairing reagent.
用SDS作对离子试剂,采用离子对反相高效液相色谱方法对反应产物进行分析。
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