The procedure of isolated microspore culture with higher regeneration was established by the technique optimization and the platform of maize DH breeding was developed.
通过技术优化，建立玉米小孢子离体培养高频再生技术程序，在此基础上构建了玉米dh育种技术平台。
Improving microspores embryogenic ability and plantlet regeneration ability from microspore-derived embryos is the key of isolated microspores culture technique.
胚胎发生能力和再生植株转化率是游离小孢子培养技术的关键问题。
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