Human embryonic fibroblast feeder played a positive role during the ES cells isolation procedure.

人胚胎成纤维细胞饲养层在ES细胞分离过程中具有较好的作用。

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Objective: To find out the extraction and isolation procedure of tigogenin which can separate a lot of tigogenin.

前言： 目的：寻找分离剑麻皂甙元的最佳工艺。

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Methods: Column chromatography was used in the isolation procedure. The structures of isolated compounds were elucidated by spectral data.

方法：柱色谱法分离其成分，利用光谱数据确定其化学结构。

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