Objective: To explore a method of detecting the connexin 43 (Cx43) quantity and distribution by laser scanning confocal microscope (LSCM) with a double label technique.
目的：探讨激光扫描共聚焦显微镜和荧光双重标记技术对心肌连接蛋白43进行定量和定位研究的方法。
METHODS Immunofluorescence histochemical double staining technique was used and the staining results were observed with confocal laser scanning microscope.
方法免疫荧光组织化学双重染色，染色结果在激光共聚焦扫描显微镜下观察。
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