...离心收获细菌细胞,裂解后将含融合蛋白的CFE加入到Talon~(TM)树脂中,进行金属离子亲和层析(Metal Ion Affinity Chromatography,MIAC),分离纯化带组氨酸标签的融合蛋白。
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...离心收获细菌细胞,裂解后将含融合蛋白的CFE加入到Talon~(TM)树脂中,进行金属离子亲和层析(Metal Ion Affinity Chromatography,MIAC),分离纯化带组氨酸标签的融合蛋白。
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immobilized metal ion affinity chromatography 金属螯合亲和层析 ; 金属离子亲和层析 ; 固相金属离子亲和色谱 ; 属离子亲和层析
Chelating-Peptide Immobilized Metal-ion Affinity Chromatography 亲和层析
Immobilized metal ion affinity chromatography sorbents were prepared for purification of reduced glutathione (GSH) using chitosan as substrate.
制备了以壳聚糖为载体的金属螯合亲和色谱分离纯化还原型谷胱甘肽(GSH)。
By immobilized metal affinity chromatography, ion-exchange chromatography and hydrophobic interaction chromatography, the recombinant Gln49-PLA2 was purified with 91% purity.
通过固定化金属亲和层析、阴离子交换、疏水层析融合蛋白得到了纯化,其纯度可达91%。
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