This review focuses on the methods of isolation and cultivation of pathogenic free-living amoebae, including sample treatment, culture conditions, passage culture, pathogen detection, and maintenance.
本文综述该病原体国内外报道的分离培养方法，主要包括样品处理、培养条件、虫株传代、虫株致病性检测和虫株保存方法。
In this paper, it reviewed a few commonly used sample pre-treatment methods and detection methods in monitoring of trace organic pollutants concentrations in water environment for the current.
本文主要针对目前监测环境水体中痕量有机污染物浓度常使用的一些样品前处理方法和检测方法进行综述。
Methods CYFRA21 1 and NSE in serum sample of 162 patients with PLC were analysed by ELISA in two weeks before and after treatment of operation, radiotherapy and chemotherapy.
方法分别在手术，放疗或化疗前后2周内采集PLC病人血清标本162份。采用酶联免疫分析方法检测标本血清中CYFRA2 1 1和nse的表达情况。
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