当时chambers i等将敲除掉lif受体基因的es细胞放入小鼠胚胎成纤维饲养层(mouse embryonic fibroblast feeder, mef feeder)细胞中培养,发现了很多小的未分化的细胞群。
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To observe the mouse blastocyst growth behavior on primary mouse embryonic fibroblast feeder layers (PMEF).
观察鼠囊胚在小鼠原代胎儿成纤维细胞饲养层上的生长行为。
Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast (MEF) cells for isolation and culturing of embryonic stem (es) cells.
目的:建立ICR小鼠胚胎成纤维细胞饲养层培养体系,用于胚胎干细胞建系及研究。
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