The optimal fermentation medium and conditions of mutant strain NAF-032 with a higher and stable yield of L-lactic acid were studied by orthogonal design theory.
运用正交试验设计理论，对高产L-乳酸突变株NAF-032的最佳摇瓶发酵条件进行了研究，确定了最佳的发酵培养基和发酵条件。
The fed-batch fermentation method was studied by flask culture based on the optimal medium.
然后在该培养基配方的基础上进行摇瓶分批补料发酵试验，确定最佳补料方案。
The results indicated that the second fermentation in PDY medium with 10% incubating volume is the optimal flask fermentation.
对发酵过程中相关因子研究，结果表明，摇瓶发酵培养宜采用10%接种量，在PDY基质上进行二级发酵。
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