②基因枪法 又称 粒子轰击 ( particle bombardment ),它是利用高压氦气或氮气将吸附重组DNA 的金粉或钨粉加速到300 m/s,高速轰击植物细胞,通过选择培养筛选转化体,该方法转化...
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应用基因枪(particle bombardment)转化技术成功将SNRGl,SNRGmL和SNRGmK导入粳稻模式品种台北309的胚性愈伤组织中,并由此获得了再生植株和细胞株。
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alpha particle bombardment 粒子轰击
Particle Bombardment Technology 微粒轰击技术
biolistic particle bombardment 生物溶解颗粒导弹方法
charged particle bombardment 荷电粒子轰击 ; 带电粒子轰击
particle bombardment-mediated gene transfer 颗粒轰击介导的基因转移
It was suggested that these promoters might be related to plant stress resistance. The promoters driving the beta-glucuronidase (GUS) and green fluorescent protein (GFP) fusion protein were transformed to maize coleoptile using particle bombardment.
为了了解上述启动子的启动强度和表达特异性,构建了带有gus和gfp融合基因的植物表达载体,利用基因枪法轰击玉米胚芽鞘进行瞬时表达分析。
参考来源 - 玉米胁迫相关基因的启动子克隆和多基因表达载体的构建The original transformation model for the seaweed Laminaria japonica (Phaeophyceae) employed female gametophytes as micro-particle bombardment targets and parthenogenesis as a route to produce transgenic parthenosporophytes.
海带遗传转化模型是以基因枪转化海带雌配子体,转化后诱导生成孤雌生殖孢子体。
参考来源 - 海带遗传转化模型的优化及目的基因的导入·2,447,543篇论文数据,部分数据来源于NoteExpress
The LEAFY gene was also introduced into cells of sugarcane calli using PDS-1000 he particle bombardment.
本研究同时以甘蔗心叶愈伤组织为受体,通过PDS - 1000氦气基因枪轰击,将LEAFY基因转入甘蔗细胞。
By particle bombardment method, 167 regenerated plantlets were gained from 1160 calli, and the transformation frequency was 17.4%.
通过基因枪转化法轰击1160块的愈伤组织得到167株再生植株,转化率17.4%。
The direct gene transfer systems involved the chemical method (PEG), electroporation, microinjection, particle bombardment, pollen-mediated gene transfer and other systems using various vectors.
阐述了化学导入法(PEG)、电穿孔法、注射法,粒子轰击法、花粉管萌导法及使用其它载体系统的成功实例。
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