By particle bombardment method, 167 regenerated plantlets were gained from 1160 calli, and the transformation frequency was 17.4%.
通过基因枪转化法轰击1160块的愈伤组织得到167株再生植株，转化率17.4%。
The direct gene transfer systems involved the chemical method (PEG), electroporation, microinjection, particle bombardment, pollen-mediated gene transfer and other systems using various vectors.
阐述了化学导入法(PEG)、电穿孔法、注射法，粒子轰击法、花粉管萌导法及使用其它载体系统的成功实例。
应用推荐