Objective: (1) to establish PCR reaction system that USES allele-specific primer PCR technique to detect SNP of KLOTHO gene.
目的：(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
Test results are as follows: (1) Potato PCR amplification reaction system and procedure are be fixed by optimizing PCR reaction system and selecting suitable primers.
试验过程得到如下结果：(1)对马铃薯P CR反应体系进行了优化，筛选出合适的引物，确定马铃薯P CR反应体系和扩增程序。
This real-time PCR reaction system is highly specific, reliable, high sensitivity for detecting pathogenic fungus which could be useful for the diagnosis of clinical fungus infection.
建立的实时荧光PCR方法能特异、灵敏的检测临床病原真菌，为临床真菌感染的诊断提供了一种快速、准确的方法。
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