方法采用PCR体外定点突变技术(PCR site-directed mutagenesis,PCR-SDM),设计4对引物,引入5个Furin识别的突变位点,通过重叠延伸法PCR扩增,使人胰岛素原编码基因B1...
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Methods The polyadenylation signal-deficient retrovirus vector mutated by PCR site-directed mutagenesis was used to make polyadenylation signal-deficient retroviruses by PA317 packaging cells.
方法使用人工定点突变多聚腺苷酸化信号的小鼠逆转录病毒载体，应用PA317病毒包装细胞获得多聚腺苷酸信号缺陷的重组逆转录病毒；
AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
Human brain derived neurotrophic factor gene was used as template for oligonucleotide based site directed mutagenesis and PCR.
应用寡核苷酸诱导的定点突变和PCR技术对人脑源性神经营养因子基因进行突变，并完成了测序鉴定。
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