对于接受化疗后自身外周血干细胞移植(peripheral blood stem cell transplant,PBSCT)的患者,其移植物污染可能导致远期复发。
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DNA samples of related hematopoietic stem cell transplant donor-recipients were extracted from peripheral blood cells. HLA-A loci were typed both by RSCA and PCR-SSP.
然后分别提取84例拟进行造血干细胞移植病人及家系成员的外周血d NA,采用RSCA和序列特异性引物体外基因扩增(PCR ssp)法平行对照对HLA A基因位点进行分型。
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