Two dimensional polyacrylamide gel electrophoresis(2D PAGE) followed by mass spectrometry(MS) is the most widely used method of protein resolution and identification. But it exist shortcomings.
双相聚苯稀酰氨凝胶电泳（2 D PAGE）与质谱（MS）是分辨和鉴定蛋白的最常用的方法，但也存在有缺点。
And proteomics technology based on two-dimensional electrophoresis is an effective approach to discover the changes in the states of protein phosphorylation.
以二维电泳为基础的蛋白质组学是发现蛋白磷酸化状态改变的有效途径。
Objective To explore an optimal cartilage protein extraction approach that can guarantee both the image quality and the result fidelity of the two-dimensional gel electrophoresis (2-DE) technique.
目的 探索可兼顾双向电泳凝胶图像质量和结果保真性的软骨蛋白提取方案。
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