技术文摘 关键词：α-淀粉酶，活性位点，随机突变，空间构象，氢键 [gap=983]Key words: α-amylase; active site; random mutagenesis; 3-D structure; H-bonds
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localized random mutagenesis 定域随机诱变
Diversify PCR Random Mutagenesis Kit 聚合酶链抗应试剂盒多样化随机突变
random mutagenesis PCR 致错PCR
random mutagenesis library 随机突变体库
random oligonucleotide mutagenesis 随机寡核苷酸诱变
The coding region of alkaline protease gene and its 3’ flanking terminator region from B. pumilus were subjected to random mutagenesis by error-prone PCR.
采用易错PCR方法对碱性蛋白酶基因编码区和终止子片段进行随机突变。
参考来源 - 脱毛蛋白酶产生菌的选育及其碱性蛋白酶基因的克隆与表达In order to improve the characterize of the Penicillum expansum lipase (PEL), the PEL gene was mutated by site-directed mutagenesis and random mutagenesis using error-prone PCR technique, and the results were showed as follow.1.
本研究利用定点突变和随机突变的技术手段。
参考来源 - 扩展青霉脂肪酶的定点突变及随机突变·2,447,543篇论文数据，部分数据来源于NoteExpress
Many research groups successfully rely on whole-gene random mutagenesis and recombination approaches for the directed evolution of enzymes.
许多研究小组利用整基因随机突变形成和重组方法，已成功的进行了酶的定向进化。
The principle and the enzymatic method for random-site-specific mutagenesis of ginseng peptide gene in vitro were reported.
本文报道了酶法体外随机——定位诱变人参多肽基因的原理和方法。
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