实时荧光定量PCR(Real-time PCR)即通过荧光染料或荧光标记的特异性探针对 PCR反应过程进行实时监测,根据PCR指数扩增期间荧光信号的变化来即时测定对应特异产物的...
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方法:荧光实时定量聚合酶链反应(real-time PCR)法扩增22例肝癌及相应癌旁组织中线粒体 DNA编码基因细胞色素C氧化酶Ⅱ(COⅡ),以核编码基因磷酸甘油醛脱氢酶(GAPDH)作为内参
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real time PCR 实时定量PCR ; 实时荧光定量PCR ; 荧光定量PCR仪 ; 荧光PCR
fluorescent Real-time PCR 荧光定量PCR ; 实时荧光PCR
Real-time PCR Detection System 测系统
dual real-time pcr 双重实时荧光pcr ; 双重荧光PCR
duplex real-time PCR 聚合酶链反应
IBD Real-Time PCR KIT 传染性法氏囊
The transcription of IL-1β, IL-10, IL-12 p40 and IL-18 mRNAs was evaluated by real-time PCR.
用实时PCR检测IL-1β、IL-10、IL-12p40和IL-18 mRNA的表达。
参考来源 - 氧化低密度脂蛋白诱导泡沫细胞形成过程中细胞因子水平的变化Optimizing the real-time PCR system,the standard curve was created. The reproducibility was also verified.
方法:构建质粒标准品,设计实时荧光PCR探针引物,优化体系,建立标准曲线,并进行重复性验证。
参考来源 - 枣疯病植原体实时荧光定量PCR检测方法的研究This dissertation describes real-time PCR with different solutions (displacing probes, ARMS primers, PAP primers and HRM) to test polymorphisms.
本论文凭借实时PCR技术平台,利用双链荧光置换探针、ARMS引物、PAP引物、高分辨溶解曲线分析等不同策略,检测不同的突变。
参考来源 - 实时荧光PCR在基因分型与稀有突变检测中的应用In the first part, the objective is to design a real-time PCR test to detect porcine endogenous retrovirus (PERV) RNA in the medical devices in which the materials derived from porcine.
第一部分的目的是设计实时荧光PCR实验检测猪源性医疗器械中猪内源性逆转录病毒(porcine endogenous retrovirus,PERV)RNA。
参考来源 - 猪源性医疗器械中猪内源性逆转录病毒的检测方法研究及白蛋白生物胶的研制·2,447,543篇论文数据,部分数据来源于NoteExpress
Moreover, to detect the mitochondria DNA copy Numbers of sample with real-time PCR.
用实时定量pcr检测标本线粒体dna的拷贝数。
To explore the method of preparation for Hantavirus s segment standard plasmids of real-time PCR.
构建汉坦病毒S片段标准品,用于实时荧光定量pcr检测汉坦病毒。
Objective To quantitatively analyse the relationship between uncultivated phylotype AU126 and periodontal disease with real-time PCR.
目的运用实时P CR技术,对未获培养微生物au126与牙周病的关系进行定量分析。
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