Supercoiling is necessary for the expression of recombinant plasmid DNA at transcription level.
超螺旋化对于质粒dna在转录水平上的表达是必需的。
Objective To construct a recombinant plasmid containing P30 gene of Toxoplasma gondii and obtain recombinant protein Methods P30 gene fragment was amplified from genomic DNA of T.
目的构建表达P30蛋白抗原表位的重组质粒及工程菌，获得纯化的P30蛋白抗原表位，用于检测弓形虫特异性抗体。
After co-transfection of recombinant plasmid and wild type AcNPV DNA, the rAcNPV was confirmed by the end point dilution assay and dot blot.
经同源重组后，以终点稀释和斑点杂交法筛选重组杆状病毒；
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