Methods a new compound was isolated by the silica gel and Sephadex LH-20 column chromatograph, and identified by the physicochemical characters and spectroscopic analysis.
方法采用硅胶柱色谱、凝胶柱色谱进行分离，通过理化性质和波谱分析方法鉴定化合物结构。
Biliary protein was isolated and purified by ammonium sulfate fraction and Sephadex G-75 gel filtration column chromatograph from complex pig bile.
采用了凝胶过滤结合硫酸铵沉淀法从异常复杂的胆汁中成功分离出一种蛋白质。
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