定点突变技术(site-directed mutagenesis)是通过分子克隆手段定点改变特定基因的局部核苷酸序列,通常被用来研究蛋白质的功能、结构,以及用于目的蛋白质的改造,如增加目的蛋...
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5． 基因的定点诱变（site-directed mutagenesis） 应用Promega PolyAT tract mRNA Isolation System分离Poly(A)RNA。
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site directed mutagenesis 定点突变 ; 定向诱变 ; 的基因定点诱变技术
site-directed mutagenesis kit 定点突变试剂盒
Application of Site-directed Mutagenesis 定点诱变技术的应用
pcr site-directed mutagenesis pcr定点突变 ; 体外定点突变技术
site-directed mutagenesis detail 定点诱变
pcr mediated site directed mutagenesis pcr定位诱变
Transformer Site-Directed Mutagenesis Kit 变压器定点突变试剂盒
amap multi site-directed mutagenesis kit A马帕多定点突变试剂盒
The above results suggested that codon usage optimization and site-directed mutagenesis technology could be utilized to improve the expression level of pro-transglutaminase in E. coli.
上述研究结果表明密码子优化及定点突变技术可以应用于优化转谷氨酰胺酶酶原在大肠杆菌中的表达。
参考来源 - 转谷氨酰胺酶酶原在大肠杆菌中的重组优化表达ConclusionsGene Furin K5 enzymatic hydrolysis of recognition sites for site-directed mutagenesis to enhance adenovirus-mediated expression of K5 protein secretion for further to adenovirus vector carrying the gene therapy K5 retinal neovascularization dysplasia diseases laid the foundation for experimental Study.
结论对K5基因Furin蛋白酶水解识别位点进行定点突变能提高腺病毒介导的K5蛋白分泌表达量,为进一步以腺病毒为载体携带K5基因治疗眼底新生血管异常增生性疾病的实验研究奠定了基础。
参考来源 - 携带Kringle5基因的重组腺病毒抗新生血管形成的实验研究Construction of L60V and I97L mutants and recombinant adenovirusesBy site-directed mutagenesis technique, p3.8llplasmid, which contains 1.2 copies of HBV genome, was used as template to produce the two desired mutations.
1．重组腺病毒的构建 用定点诱变技术，以含有1.2拷贝HBV基因组的p3.8Ⅱ质粒为模板，构建含L60V和I97L的突变株。
参考来源 - 用腺病毒载体介导的基因转移研究HBV/C区热点变异对小鼠免疫应答的影响·2,447,543篇论文数据，部分数据来源于NoteExpress
以上来源于: WordNet
Site-directed mutagenesis is one of the key methods used to study gene function.
定点突变是研究基因功能的一种关键方法。
Objective: To develop a simple and efficient way to perform site-directed mutagenesis.
目的：介绍一种简便、有效的定点突变技术。
In the past, the Affinity Labeling and Site-Directed Mutagenesis are the primary methods to study amine receptor.
过去主要是通过亲和标记和点突变等实验方法进行研究。
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