..., 定点饱和突变, 双突变, 定点突变 [gap=1756]Keywords: Coptotermes formosanus, endoglucanase, site-saturation mutagenesis, combined mutation, site-directed mutagenesis ..
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By site-saturation mutagenesis, the effects of different amino acids in the conservative site on the characteristics of enzymes were investigated. The research provided the theories basis for the further molecular modification of glycerol dehydratase.
甘油脱水酶(glycerol dehydratase,EC 4.2.1.30)是微生物合成1,3-丙二醇(工业上有重要用途的化工材料)途径中的限速酶,本研究通过定点饱和突变的方法研究甘油脱水酶保守位点上的不同氨基酸对酶学性质的影响,为进一步对甘油脱水酶的活性中心进行分子改造提供理论依据。
参考来源 - Klebsiella pneumoniae甘油脱水酶α亚基Ile498的分子改造·2,447,543篇论文数据,部分数据来源于NoteExpress
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