Aim: To develop a PCR technique for rapid screening of recombinant plasmid in subtractive library of cDNA.
目的：消减文库构建过程中，用P CR技术快速筛选重组阳性克隆。
Recombinant clone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
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