Methods: color reaction of free amino acids with ninhydrin was adopted, since, in certain extent, the color depth is correlated with the amino acid content.
方法:采用氨基酸的游离氨基与水合茚三酮产生显色反应,在一定范围内,其颜色的深浅与氨基酸的含量成正比。
The surface amino group was transferred to the aldehyde group after it reacted with ninhydrin. The surface aldehyde group was confirmed by the mirror reaction.
表面的伯胺基在与茚三酮反应后转化为醛基,通过银镜反应证明了醛基的存在。
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